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normal lung fibroblast cells wi38  (ATCC)


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    ATCC normal lung fibroblast cells wi38
    Normal Lung Fibroblast Cells Wi38, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 3624 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/normal lung fibroblast cells wi38/product/ATCC
    Average 99 stars, based on 3624 article reviews
    normal lung fibroblast cells wi38 - by Bioz Stars, 2026-03
    99/100 stars

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    HCT116 isogenic panel treated with CB002 or analog #4 for 48 hr and their respective IC50 values shown in the table ( A ). CB002-analog #4 increases apoptotic cells as indicated by the sub-G1 content in cancer cells but not in normal <t>WI38</t> cells (48 hr). Two-way ANOVA, p<0.0001 ( B ). 72 hr treatment with CB002-analog #4 is most potent ( C ) and increases dead cells as indicated by the ethidium homodimer staining (red) compared to calcein stained live cells (green) ( A ), and cleaved caspase-3 (green) immunofluorescence ( D ) in colorectal cancer patient-derived organoid cells. CB002-analog #4 decreases ki67 staining (green) in a dose-dependent manner (72 hr) in colorectal cancer patient-derived organoid cells ( E ). CB002-analog #4 is non-toxic in vivo ( F ) and significantly reduces tumor volume in NSG mouse xenografts with SW480 wild-type cells ( G ) but not in SW480 cells with shNoxa ( H ). 50 mg/kg by oral gavage three times per week, final tumor volume at 5 weeks. Unpaired t-test, p<0.05.
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    HCT116 isogenic panel treated with CB002 or analog #4 for 48 hr and their respective IC50 values shown in the table ( A ). CB002-analog #4 increases apoptotic cells as indicated by the sub-G1 content in cancer cells but not in normal <t>WI38</t> cells (48 hr). Two-way ANOVA, p<0.0001 ( B ). 72 hr treatment with CB002-analog #4 is most potent ( C ) and increases dead cells as indicated by the ethidium homodimer staining (red) compared to calcein stained live cells (green) ( A ), and cleaved caspase-3 (green) immunofluorescence ( D ) in colorectal cancer patient-derived organoid cells. CB002-analog #4 decreases ki67 staining (green) in a dose-dependent manner (72 hr) in colorectal cancer patient-derived organoid cells ( E ). CB002-analog #4 is non-toxic in vivo ( F ) and significantly reduces tumor volume in NSG mouse xenografts with SW480 wild-type cells ( G ) but not in SW480 cells with shNoxa ( H ). 50 mg/kg by oral gavage three times per week, final tumor volume at 5 weeks. Unpaired t-test, p<0.05.
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    HCT116 isogenic panel treated with CB002 or analog #4 for 48 hr and their respective IC50 values shown in the table ( A ). CB002-analog #4 increases apoptotic cells as indicated by the sub-G1 content in cancer cells but not in normal WI38 cells (48 hr). Two-way ANOVA, p<0.0001 ( B ). 72 hr treatment with CB002-analog #4 is most potent ( C ) and increases dead cells as indicated by the ethidium homodimer staining (red) compared to calcein stained live cells (green) ( A ), and cleaved caspase-3 (green) immunofluorescence ( D ) in colorectal cancer patient-derived organoid cells. CB002-analog #4 decreases ki67 staining (green) in a dose-dependent manner (72 hr) in colorectal cancer patient-derived organoid cells ( E ). CB002-analog #4 is non-toxic in vivo ( F ) and significantly reduces tumor volume in NSG mouse xenografts with SW480 wild-type cells ( G ) but not in SW480 cells with shNoxa ( H ). 50 mg/kg by oral gavage three times per week, final tumor volume at 5 weeks. Unpaired t-test, p<0.05.

    Journal: eLife

    Article Title: A subset of CB002 xanthine analogs bypass p53-signaling to restore a p53 transcriptome and target an S-phase cell cycle checkpoint in tumors with mutated-p53

    doi: 10.7554/eLife.70429

    Figure Lengend Snippet: HCT116 isogenic panel treated with CB002 or analog #4 for 48 hr and their respective IC50 values shown in the table ( A ). CB002-analog #4 increases apoptotic cells as indicated by the sub-G1 content in cancer cells but not in normal WI38 cells (48 hr). Two-way ANOVA, p<0.0001 ( B ). 72 hr treatment with CB002-analog #4 is most potent ( C ) and increases dead cells as indicated by the ethidium homodimer staining (red) compared to calcein stained live cells (green) ( A ), and cleaved caspase-3 (green) immunofluorescence ( D ) in colorectal cancer patient-derived organoid cells. CB002-analog #4 decreases ki67 staining (green) in a dose-dependent manner (72 hr) in colorectal cancer patient-derived organoid cells ( E ). CB002-analog #4 is non-toxic in vivo ( F ) and significantly reduces tumor volume in NSG mouse xenografts with SW480 wild-type cells ( G ) but not in SW480 cells with shNoxa ( H ). 50 mg/kg by oral gavage three times per week, final tumor volume at 5 weeks. Unpaired t-test, p<0.05.

    Article Snippet: DLD-1 (p53 S241F ) (RRID: CVCL_0248 ), SW480 (p53 R273H,P309S ) (RRID: CVCL_0546 ), and HCT116 (p53 WT ) (RRID: CVCL_0291 ) colorectal cancer cell lines and WI38 normal lung fibroblast cells were purchased from ATCC.

    Techniques: Staining, Immunofluorescence, Derivative Assay, In Vivo